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Crown Scientific cells are the only human cells tested for Purity and Immune Potency!

Our patent-pending potency test includes human immune-regulation of both adaptive and innate immune system

Primary cells are identified for purity of MSC based on ISCT standards:

- Plastic Adherence (spindle morphology)

Immunophenotyping:

- Positive (>90%): CD105, CD90, CD73, CD29, CD44

- Negative (<10%): CD45, CD34, CD19, CD14, CD11b, CD79a, HLD-DR

Antibody	Population (%)
CD105	+++
CD73	+++
CD90	+++
CD29	+++
CD44	+++
CD45	-
CD34	-
CD14	-
CD79a	-
CD11b	-
HLADR	-

- Tri-lineage differentiation*: Adipocyte, Chondrocyte, Osteocyte

Our cells are tested for Immune regulation potency (Anti-inflammatory effect): Potency assays are performed in order to evaluate and make sure that the potency to cause immune-supression is adequate in every released batch. The immune system can be divided in two: the adaptive (acquired immunity) and innate (first line of defense), at Crown Scientific we test the potency of our MSCs to regulate both immune systems:

- Adaptive Immune System inhibition (IC50):