Research

At Crown Scientific we carry multiple scientific projects with different maturity levels, some of the main projects we are scientifically involved are:

 

1) The use of human MSCs cells to treat autoimmune, chronic inflammation, and fibrosis.

 

 

More than 80 diseases occur as a result of the immune system attacking the body’s own organs, tissues, and cells. Immune-related dysfunctions are a paramount medical problem of this century. At Crown Scientific we have been study the immune regulatory effects of MSCs, mainly from perinatal tissues and bone marrow. We have isolated and compared the therapeutic effect of various MSCs  and compared them based on their immune regulatory functionality in vitro and in vivo. With the simple goal of answering: a) is there a difference between MSCs tissue sources in their therapeutic effect? b) if there is a difference, which source is more potent and robust?

 

The regenerative medicine field, in particular MSCs therapeutics, hence we decided to use functional assays and let the data speak for itself. Below an example of in vivo functional data tested by our scientific team:

 

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At Crown Scientific our mission is to find cures for autoimmune diseases, with a focus on cell therapeutics and regenerative medicine. With the immune system in mind, we have developed a battery of functional assays and animal models to evaluate, compare, and discover novel biological therapeutics that offer innovative solutions to incurable diseases.

 

 

2) Cell-free therapeutics for treatment of chronic inflammation and autoimmunity.

 

At Crown Scientific we have identified at least one Mechanism Of Action (MOA) of MSCs and we have been able to extract a cell-free product capable of having a therapeutic effect similar to that of whole cells. This cell free therapeutic can be produced in a cost effective matter and can be applied at repeated times in animal models and its immune regulatory potency has been tested. We are currently working on strategies to start human clinical trials.

 

In vitro result:

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Higher T cell proliferation --> higher inflammation; the goal is to reduce T cell proliferation that to demonstrate beneficial bioactivity of the product.
MSCs are capable of inhibiting human T cells by 85%.
Cell Free product (CM1) has similar T cell inhibitory effect than using complete cells.
Commercial Cell Free product does not inhibit T cells at the concentration tested.
Summary: The CM1 has the capability (bioactivity) similar to the cells without having to use cells. Reducing the risk and hurdles of using cryopreserved viable cells

 

In vivo result:

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This is a humanized animal model for Graft versus Host Disease. Mice without an immune system gets injected with human peripheral blood mononucleated cells (PBMC) and the human immune system starts attacking the mice tissues. The higher the GvHD score, the more progression of GvHD.
A survival curve shows that by day 50, half of the control (GvHD) mice have died while 100% of the CM (cell-free) group is still alive (on-going experiment).

 

 

Clinical GvHD scores were significantly reduced and survival was significantly increased using the cell free product in a humanized graft versus host disease model.

 

 

3) Exosomes derived from activated perinatal stromal cells as inhibitor of ovarian cancer cell proliferation.

Exosomes are a new and exciting means of delivering treatment to the human body, and our project and product are designed to harness the therapeutic potential of exosomes. If research and development are successful, our product will provide women with a safe and effective treatment for ovarian cancer.

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Effect of exosomes from activated perinatal MSCs on ovarian cancer cell line. Ovarian cancer cell line proliferation in vitro. Panel A) no treatment. Panel B) exosome-treated at 72 hrs. 

 

 4) Thin film coatings for biomaterials and biomedical applications

Metallic materials such as titanium alloys, zirconium and sst 314 are biocompatible but have little corrosion resistance in presence of aggressive body fluid. They have good mechanical properties such as fatigue and tensile strength but are vulnerable when they are in contact with other components because of friction. Thin film ceramic coating is promising technique to enhance such properties. In our research center we provide physical vapor deposition (PVD), Anodization, and Electrodeposition services to solve the corrosion and wear resistance of biomaterials.

 

 5) Biosensor for glucose detection

In our research center we’ve been using noninvasive techniques to fabricate reliable wearable wireless sweat base electrochemical and optical sensors for glucose detection and monitoring. Combined with the vigorous patient monitoring systems and devices, the wearable wireless biosensors can bring clinicians with better peace of mind.

 

Collaborators: Novothelium, UTSA, Baylor College of Medicine, Pall Corporation, Sigma-Aldrich